{"created":"2023-05-15T14:59:40.760703+00:00","id":314,"links":{},"metadata":{"_buckets":{"deposit":"6bd6b0a5-ceb4-43a2-a297-2c6dd6a9f33d"},"_deposit":{"created_by":9,"id":"314","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"314"},"status":"published"},"_oai":{"id":"oai:kamakura-wu.repo.nii.ac.jp:00000314","sets":["1:52"]},"author_link":["1046","1042","1045","1044","1047","1043"],"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"14","bibliographicPageEnd":"23","bibliographicPageStart":"13","bibliographic_titles":[{"bibliographic_title":"鎌倉女子大学紀要"},{"bibliographic_title":"The journal of Kamakura Women's University","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"3, 3', 4', 5-tetrachlorosalicylanilide(TCSA)によって光接触過敏症(contact photosensitivity;CPS)を誘発したマウスの脾細胞を、未処置マウスに細胞移入した。細胞移入24時間後に、マウスの耳介部にTCSAでCPS反応を惹起した結果、通常のTCSAによるCPSと同レベルの耳介腫脹反応が認められた。次に、TCSAにより光感作誘導処置を行ったマウスを用いて、UVA照射済み光ハプテン(TCSA)塗布および、TCSAを塗布した後にUVA照射することでCPS反応を惹起し、24時間後の耳介腫脹および反応部位(耳介部)に発現するサイトカインをRT-PCR法で解析を行った。その結果、両群とも耳介腫脹は顕著に認められ、それに加えてIL-4mRNA発現も同程度に認められた。これらの結果から、TCSAによって誘導されるCPSは細胞移入により誘発が可能な系であること、並びに、あらかじめUVA照射したTCSAで惹起できることが明らかになった。そこで次に、TCSAによる光感作誘発マウスの脾細胞を用いてCPS反応を、in vitroにおいてTh2型サイトカインの産生を指標に検討を行った。光ハプテン(TCSA)を添加後UVA照射を施す群(I群 : いわゆる、通常の光感作群)、UVA照射済み光ハプテン(TCSA)を細胞に添加する群(II群)および細胞にUVA照射を施した後、光ハプテン(TCSA)を添加する群(III群)の3群構成で、各々の群の培養上清中のサイトカイン量をELISAにて測定した。その結果、特にI群とII群においてIL-4及びIL-5の産生量が上昇傾向にあり、Th2型サイトカインがCPSの反応成立の際に重要であるという過去の実験データと一致した。以上の結果から、Th2型サイトカインの産生を指標にして、光感作アレルギー物質(光アレルゲン)のin vitroによるスクリーニングがある程度可能であることが示された。"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Splenic cells from mice that had induced contact photosensitivity (CPS) by treatment with 3, 3', 4', 5-tetrachlorosalicylanilide (TCSA) were transplanted to untreated naive mice. The CPS reaction was elicited with TCSA in the auricle of the mice 24 hours after the cell transplant. Ear-swelling, equivalent to that of the mice photosensitized with TCSA, was observed. CPS reaction was elicited in photosensitized mice either by applying UVA-irradiated photo hapten (TCSA) or by applying TCSA, then irradiating with UVA after application. 24 hours later, ear-swelling was checked and cytokine expression in the lesion was analyzed using RT-PCR. Significant ear-swelling and IL-4 mRNA expression were observed in both groups. These test results indicate that the TCSA-triggered CPS can be induced by cell transplantation, and that the CPS can be elicited by UVA-radiated TCSA. CPS reaction was then studied in vitro with the total spleen cells from the mice photosensitized by TCSA, using Th2 cytokine production as the indicator. The study was conducted on 3 groups of cells. For group I, photo hapten (TCSA) was added to the cells and then UVA irradiation is performed (regular photosensitive group); for group II, UVA-treated photo hapten (TCSA) was added to the cells; for group III, photo hapten (TCSA) was added to the UVA-treated cells. For each group, the cytokine in culture supernatant was measured by ELISA. IL4 and IL5 production increased in group I and II supporting the previously obtained data suggesting that Th2 cytokine plays an important role in CPS reaction. Our study indicates that in vitro screening of photo allergen using Th2 cytokine production as an indicator is effective to some extent."}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10434900","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09199780","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山崎, 俊介"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 真由"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安藤, 博文"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamazaki, Shunsuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Mayu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Andou, Hirofumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-30"}],"displaytype":"detail","filename":"KJ00004699626.pdf","filesize":[{"value":"764.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"KJ00004699626.pdf","url":"https://kamakura-wu.repo.nii.ac.jp/record/314/files/KJ00004699626.pdf"},"version_id":"41aa8095-6dcb-41fd-92b1-8828841181e1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"光接触過敏症"},{"subitem_subject":"サイトカイン"},{"subitem_subject":"脾細胞"},{"subitem_subject":"Contact photosensitivity"},{"subitem_subject":"Cytokines"},{"subitem_subject":"Spleen cells"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_title":"光感作マウスの脾細胞を用いた光アレルギー反応のIn Vitroスクリーニング法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"光感作マウスの脾細胞を用いた光アレルギー反応のIn Vitroスクリーニング法の開発"},{"subitem_title":"Development of In Vitro Screening System for Photo Allergen Using Splenic Cells from Photosensitized Mice"}]},"item_type_id":"3","owner":"9","path":["52"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-30"},"publish_date":"2017-05-30","publish_status":"0","recid":"314","relation_version_is_last":true,"title":["光感作マウスの脾細胞を用いた光アレルギー反応のIn Vitroスクリーニング法の開発"],"weko_creator_id":"9","weko_shared_id":-1},"updated":"2023-05-15T15:22:17.985438+00:00"}